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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC613.01
Gene ID 24/B07
Gene synonyms/obsolete SPBC757.14; SPCC757.14
Gene product transporter; unknown specificity; similar to Sp SPCC613.02 and SPCC330.07c and SPCC757.11c (paralogs); conserved in A. thaliana; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 1494
ORF length (spliced)
Entry clone length 1494
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments Not analyzed correctly (sequence_seqL2).
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC613.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGCACTACGACCGAAAC
Rev primer name SPCC613.01.Rv
Rev primer SEQ AGAAAGCTGGGTAACCAGTCTTGGTTTTAATA
Amino acid length 497
Molecular weight 55.1
Isoelectric point (calc.) 9.5
Signal SEQ
No. of transmembrane domain 12
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) periphery; cytoplasmic dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 2 images) See all images

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