Gene name |
SPBC2D10.09 |
Gene ID |
22/D03 |
Gene synonyms/obsolete |
|
Gene product |
3-hydroxyisobutyryl-coenzyme a hydrolase; Enoyl-CoA
isomerase family |
Entry clone |
Cloned |
ORF length (unspliced) |
1290 |
ORF length (spliced) |
|
Entry clone length |
1290 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC2D10.09.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGATTGAAATTAAATAT |
Rev primer name |
SPBC2D10.09.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATAAATAAGGATAAGTCTTA |
Amino acid length |
429 |
Molecular weight |
47.9 |
Isoelectric point (calc.) |
9.7 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LKTSVSNFLSL/LPGYFGTYLGL |
Localization (YFP) |
no apparent
signal |
Comments for localization |
|
Effect of LMB on protein
localization |
not determined |
Microscope used for
observation |
Confocal
|