Gene name |
SPBC428.11 |
Gene ID |
22/D02 |
Gene synonyms/obsolete |
|
Gene product |
homocysteine synthase;
non-essential; involved in sulfur metabolism
(regulation) |
Entry clone |
Cloned |
ORF length (unspliced) |
1290 |
ORF length (spliced) |
|
Entry clone length |
1290 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
(-12)G:deletion /
851T:C |
Comments |
ApaI or SacII should
be used instead of NotI for integration using pDUAL. 5'
terminus is frameshifted. |
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC428.11.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCAGTCGAGAGTGAACA |
Rev primer name |
SPBC428.11.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGGCAACTTCCAAAGCTTGA |
Amino acid length |
429 |
Molecular weight |
46.4 |
Isoelectric point (calc.) |
6.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLQGLETLSL |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
except nucleolus |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |