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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC3C7.09
Gene ID 22/D01
Gene synonyms/obsolete
Gene product histone lysine methyltransferase; SET domain; similar to Sp SPBC1709.13C
Entry clone Cloned
ORF length (unspliced) 1290
ORF length (spliced)
Entry clone length 1290
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC3C7.09.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGATATCAAATACAATGA
Rev primer name SPAC3C7.09.Rv
Rev primer SEQ AGAAAGCTGGGTATGACTCAATGGTCTGGCTA
Amino acid length 429
Molecular weight 49.5
Isoelectric point (calc.) 5.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization mixture with 'nucleus>cytosol' localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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