Gene name |
SPCC736.03c |
Gene ID |
22/D04 |
Gene synonyms/obsolete |
|
Gene product |
phenylalanyl-tRNA
synthetase; involved in phenylalanyl-tRNA aminoacylation;
phenylalanine-tRNA ligase activity |
Entry clone |
Cloned |
ORF length (unspliced) |
1290 |
ORF length (spliced) |
|
Entry clone length |
1290 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
160A:G / 1202A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC736.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTCCCTCATCGAGTCTC |
Rev primer name |
SPCC736.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATCTGAGCTCGACTCTCAAA |
Amino acid length |
429 |
Molecular weight |
50.1 |
Isoelectric point (calc.) |
9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
no apparent
signal |
Comments for localization |
|
Effect of LMB on protein
localization |
not determined |
Microscope used for
observation |
Confocal
|