Gene name |
SPAC22G7.07c |
Gene ID |
19/G12 |
Gene synonyms/obsolete |
|
Gene product |
conserved
hypothetical; conserved protein; no apparent Sc ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
1242 |
ORF length (spliced) |
|
Entry clone length |
1242 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
738T:C / 821T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC22G7.07.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGACGACACTAGTTGTCT |
Rev primer name |
SPAC22G7.07.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATTGTCTGATGACCAGTAT |
Amino acid length |
413 |
Molecular weight |
47.3 |
Isoelectric point (calc.) |
8.7 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LQRVRRALQL/LGYLKALPI |
Localization (YFP) |
mitochondrion;
cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |