| Gene name |
SPBC12D12.06 |
| Gene ID |
19/G11 |
| Gene synonyms/obsolete |
|
| Gene product |
cyclin C homolog; RNA
polymerase holoenzyme component (putative) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1241 |
| ORF length (spliced) |
687 |
| Entry clone length |
1241 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC12D12.06.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCAGCAAATTACTGGGC |
| Rev primer name |
SPBC12D12.06.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTCAATATCCTCAAAATAA |
| Amino acid length |
228 |
| Molecular weight |
26.1 |
| Isoelectric point (calc.) |
5.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
no apparent
signal |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
Confocal
|