Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC9B6.03
Gene ID 11/H10
Gene synonyms/obsolete
Gene product conserved hypothetical; zinc finger protein; zf-FYVE type
Entry clone Cloned
ORF length (unspliced) 882
ORF length (spliced)
Entry clone length 882
No. of intron 0
Sequence status Finished
Sequence results 22T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC9B6.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACAACTGTTCACCAATT
Rev primer name SPBC9B6.03.Rv
Rev primer SEQ AGAAAGCTGGGTAAAAGGTACTCCAAACCCAG
Amino acid length 293
Molecular weight 32
Isoelectric point (calc.) 8.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) vacuole?
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 4 images) See all images

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