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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC19G12.13c
Gene ID 11/H09
Gene synonyms/obsolete
Gene product sequence orphan; hypothetical protein
Entry clone Cloned
ORF length (unspliced) 881
ORF length (spliced) 750
Entry clone length 881
No. of intron 2
Sequence status Finished
Sequence results 253T:C / 360T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC19G12.13.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAATGAGAAGATTCGTTC
Rev primer name SPAC19G12.13.Rv
Rev primer SEQ AGAAAGCTGGGTAATTAATGTTTGAGGTAAGC
Amino acid length 249
Molecular weight 29.7
Isoelectric point (calc.) 7.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 4 images) See all images

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