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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC162.03
Gene ID 11/H08
Gene synonyms/obsolete
Gene product short chain dehydrogenase
Entry clone Cloned
ORF length (unspliced) 879
ORF length (spliced)
Entry clone length 879
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC162.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAATCTACGTTTTCTAC
Rev primer name SPCC162.03.Rv
Rev primer SEQ AGAAAGCTGGGTACATAGATTGAAGATTTTGA
Amino acid length 292
Molecular weight 32.6
Isoelectric point (calc.) 5.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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