| Gene name |
SPBC25H2.06c |
| Gene ID |
11/H11 |
| Gene synonyms/obsolete |
hrf1 |
| Gene product |
involved in
intracellular protein transport; involved in ER to Golgi
transport; interacts physically with SPCC61.04c; complexed
with SPCC61.04c; COPII coated vesicle component |
| Entry clone |
Cloned |
| ORF length (unspliced) |
882 |
| ORF length (spliced) |
|
| Entry clone length |
882 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC25H2.06.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCACCCAAGTTGTACCA |
| Rev primer name |
SPBC25H2.06.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGACAGCACATACATGAAT |
| Amino acid length |
293 |
| Molecular weight |
33 |
| Isoelectric point (calc.) |
9.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
4 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
no apparent
signal |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
Leica |