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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC23C11.05
Gene ID 11/G01
Gene synonyms/obsolete ppa1; ppa
Gene product inorganic pyrophosphatase
Entry clone Cloned
ORF length (unspliced) 870
ORF length (spliced)
Entry clone length 870
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC23C11.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGTGAATACACTACCCG
Rev primer name SPAC23C11.05.Rv
Rev primer SEQ AGAAAGCTGGGTAAAGAGGAGAGCCAGAAATG
Amino acid length 289
Molecular weight 32.4
Isoelectric point (calc.) 4.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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