Gene name |
SPAC19A8.07c |
Gene ID |
11/G02 |
Gene synonyms/obsolete |
|
Gene product |
ribonucleoprotein
complex; processome component; involved in RNA processing; U3
snoRNP protein; eukaryotic conserved protein; Brix domain;
RNA-binding protein; involved in ribosome biogenesis and
assembly |
Entry clone |
Cloned |
ORF length (unspliced) |
870 |
ORF length (spliced) |
|
Entry clone length |
870 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC19A8.07.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCTTCGTCGTGCTGTTAG |
Rev primer name |
SPAC19A8.07.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTCAGCTAAAACATCACGT |
Amino acid length |
289 |
Molecular weight |
33.3 |
Isoelectric point (calc.) |
9.3 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleolus>>nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |