Gene name |
SPAC10F6.05c |
Gene ID |
10/C08 |
Gene synonyms/obsolete |
ubc6 |
Gene product |
ubiquitin conjugating
enzyme |
Entry clone |
Cloned |
ORF length (unspliced) |
808 |
ORF length (spliced) |
684 |
Entry clone length |
808 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
769T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC10F6.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCGTCGAAAGGTGCTTA |
Rev primer name |
SPAC10F6.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGAATCAGCTCCAAAAAAA |
Amino acid length |
227 |
Molecular weight |
25.6 |
Isoelectric point (calc.) |
9 |
Signal SEQ |
|
No. of transmembrane domain |
1 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
no apparent
signal |
Comments for localization |
|
Effect of LMB on protein
localization |
not determined |
Microscope used for
observation |
Confocal
|