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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC10F6.05c
Gene ID 10/C08
Gene synonyms/obsolete ubc6
Gene product ubiquitin conjugating enzyme
Entry clone Cloned
ORF length (unspliced) 808
ORF length (spliced) 684
Entry clone length 808
No. of intron 2
Sequence status Finished
Sequence results 769T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC10F6.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCGTCGAAAGGTGCTTA
Rev primer name SPAC10F6.05.Rv
Rev primer SEQ AGAAAGCTGGGTAAGAATCAGCTCCAAAAAAA
Amino acid length 227
Molecular weight 25.6
Isoelectric point (calc.) 9
Signal SEQ
No. of transmembrane domain 1
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) no apparent signal
Comments for localization
Effect of LMB on protein localization not determined
Microscope used for observation Confocal

Image information
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