Gene name |
SPBC16G5.03 |
Gene ID |
10/C07 |
Gene synonyms/obsolete |
|
Gene product |
zinc finger protein;
zf-C3HC4 type (RING finger); ubiquitin ligase (E3); no
apparent orthologs |
Entry clone |
Cloned |
ORF length (unspliced) |
807 |
ORF length (spliced) |
|
Entry clone length |
807 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC16G5.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAAAAGCAATTCCTTGT |
Rev primer name |
SPBC16G5.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACGAATTTTCCGTCTCAGGA |
Amino acid length |
268 |
Molecular weight |
31.3 |
Isoelectric point (calc.) |
8.6 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus; nuclear dots;
mitochondrion |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal,
DeltaVision |