Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC2A9.09
Gene ID 10/C09
Gene synonyms/obsolete
Gene product conserved phosducin-like protein; involved in pheromone response
Entry clone Cloned
ORF length (unspliced) 809
ORF length (spliced) 702
Entry clone length 809
No. of intron 2
Sequence status Finished
Sequence results 238T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC2A9.09.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAATCCTGACGAAGATAC
Rev primer name SPBC2A9.09.Rv
Rev primer SEQ AGAAAGCTGGGTAATCAAAATCATCATCCAAA
Amino acid length 233
Molecular weight 26.2
Isoelectric point (calc.) 4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) no apparent signal
Comments for localization
Effect of LMB on protein localization not determined
Microscope used for observation Confocal

Image information
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