Gene name |
SPAC26A3.03c |
Gene ID |
08/D03 |
Gene synonyms/obsolete |
|
Gene product |
hypothetical protein;
Sc NCE4 (homolog) is a negative regulator of Cts1p
expression |
Entry clone |
Cloned |
ORF length (unspliced) |
708 |
ORF length (spliced) |
|
Entry clone length |
708 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC26A3.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAATCAAACTACCACCTT |
Rev primer name |
SPAC26A3.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACGCCTTTGTTTTCGACAAT |
Amino acid length |
235 |
Molecular weight |
26.6 |
Isoelectric point (calc.) |
5.8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LKKMCRLIL |
Localization (YFP) |
no apparent
signal |
Comments for localization |
|
Effect of LMB on protein
localization |
not determined |
Microscope used for
observation |
Confocal
|