Gene name |
SPAC12B10.02c |
Gene ID |
08/D04 |
Gene synonyms/obsolete |
|
Gene product |
hypothetical protein;
sequence orphan |
Entry clone |
Cloned |
ORF length (unspliced) |
708 |
ORF length (spliced) |
|
Entry clone length |
708 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC12B10.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCGAATGTGCAACGTAA |
Rev primer name |
SPAC12B10.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATCTTCTATTTTCAAAACCA |
Amino acid length |
235 |
Molecular weight |
26.9 |
Isoelectric point (calc.) |
9.4 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
2 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LRTFVLLIL |
Localization (YFP) |
ER |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal
|