Gene name |
SPBC27.06c |
Gene ID |
03/B05 |
Gene synonyms/obsolete |
|
Gene product |
conserved hypothetical
protein; eukaryotic conserved protein |
Entry clone |
Cloned |
ORF length (unspliced) |
406 |
ORF length (spliced) |
363 |
Entry clone length |
406 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC27.06.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCAATCTATGCAACCTTC |
Rev primer name |
SPBC27.06.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGCACTCAGCAACTTCATTT |
Amino acid length |
120 |
Molecular weight |
12.9 |
Isoelectric point (calc.) |
10.5 |
Signal SEQ |
|
No. of transmembrane domain |
2 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytoplasmic dots |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal |