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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC191.08
Gene ID 03/B04
Gene synonyms/obsolete
Gene product similar to Sc YNL260C -essential protein of unknown function which localizes to cell wall
Entry clone Cloned
ORF length (unspliced) 405
ORF length (spliced)
Entry clone length 405
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC191.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGACTGGGAGGAAGTGAC
Rev primer name SPCC191.08.Rv
Rev primer SEQ AGAAAGCTGGGTACTCAAAGCCATCATTAGCA
Amino acid length 134
Molecular weight 15.4
Isoelectric point (calc.) 4.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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