研究業績

原著論文 2000-

2024

Shintomi, K., Y. Masahara-Negishi, M. Shima, S. Tane and T. Hirano. (2024). Recombinant cyclin B-Cdk1-Suc1 capable of multi-site mitotic phosphorylation in vitro. PLoS One. 19:e0299003.
PMID: 38527022
Yoshida, M. M., K. Kinoshita, K. Shintomi, Y. Aizawa and T. Hirano. (2024). Regulation of condensin II by self-suppression and release mechanisms. Mol Biol Cell. 35:ar21.
PMID: 38088875

2023

Yamamoto, T., K. Kinoshita and T. Hirano. (2023). Elasticity control of entangled chromosomes: Crosstalk between condensin complexes and nucleosomes. Biophys J. 122:3869-3881.
PMID: 37571823
Sun, M., H. Amiri, A. B. Tong, K. Shintomi, T. Hirano, C. Bustamante and R. Heald. (2023). Monitoring the compaction of single DNA molecules in Xenopus egg extract in real time. Proc Natl Acad Sci USA. 120:e2221309120.
PMID: 36917660

2022

Tane, S., K. Shintomi, K. Kinoshita, Y. Tsubota, M. M. Yoshida, T. Nishiyama and T. Hirano. (2022). Cell cycle-specific loading of condensin I is regulated by the N-terminal tail of its kleisin subunit. eLife. 11:e84694.
PMID: 36511239
Yoshida, M. M., K. Kinoshita, Y. Aizawa, S. Tane, D. Yamashita, K. Shintomi and T. Hirano. (2022). Molecular dissection of condensin II-mediated chromosome assembly using in vitro assays. eLife. 11:e78984.
PMID: 35983835
Yamazaki, H., M. Takagi, H. Kosako, T. Hirano and S. H. Yoshimura. (2022). Cell cycle-specific phase separation regulated by protein charge blockiness. Nat Cell Biol. 24:625-632.
PMID: 35513709
Kinoshita, K., Y. Tsubota, S. Tane, Y. Aizawa, R. Sakata, K. Takeuchi, K. Shintomi, T. Nishiyama and T. Hirano. (2022). A loop extrusion-independent mechanism contributes to condensin I-mediated chromosome shaping. J Cell Biol. 221:e202109016.
PMID: 35045152

2021

Shintomi, K. and T. Hirano. (2021). Guiding functions of the C-terminal domain of topoisomerase IIα advance mitotic chromosome assembly. Nat Commun. 12:2917.
PMID: 34006877

2019

Sakai, Y., T. Hirano and M. Tachikawa. (2019). Molecular dynamics simulations of condensin-mediated mitotic chromosome assembly. Methods Mol Biol. 2004:319-334.
PMID: 31147926
Hara, K., K. Kinoshita, T. Migita, K. Murakami, K. Shimizu, K. Takeuchi, T. Hirano and H. Hashimoto. (2019). Structural basis of HEAT-kleisin interactions in the human condensin I subcomplex. EMBO Rep. 20:e47183.
PMID: 30858338

2018

Shintomi, K. and T. Hirano. (2018). Reconstitution of mitotic chromatids in vitro. Curr Protoc Cell Biol. 79:e48.
PMID: 29924489
Sakai, Y., A. Mochizuki, K. Kinoshita, T. Hirano and M. Tachikawa. (2018). Modeling the functions of condensin in chromosome shaping and segregation. PLoS Comput Biol. 14:e1006152.
PMID: 29912867
Takagi, M., T. Ono, T. Natsume, C. Sakamoto, M. Nakao, N. Saitoh, M. T. Kanemaki, T. Hirano and N. Imamoto. (2018). Ki-67 and condensins support the integrity of mitotic chromosomes through distinct mechanisms. J Cell Sci. 131: jcs212092 doi: 10.1242.
PMID: 29487178

2017

Shintomi, K. and T. Hirano. (2017). Mitotic chromosome assembly in vitro: functional cross talk between nucleosomes and condensins. Cold Spring Harb Symp Quant Biol. pii: 033639. doi: 10.1101/sqb.2017.82.033639.
PMID: 29118204
Hirano, T., T. Nishiyama and K. Shirahige. (2017). Hot debate in hot springs: Report on the second international meeting on SMC proteins. Genes Cells. 22:934-938.
PMID: 29067760
Hirano, T. (2017). Capturing condensin in chromosomes. Nat Genet. 49:1419-1420.
PMID: 28951628
Ono, T., C. Sakamoto, M. Nakao, N. Saitoh and T. Hirano. (2017). Condensin II plays an essential role in reversible assembly of mitotic chromosomes in situ. Mol Biol Cell. 28:2875-2886.
PMID: 28835373
Shintomi, K., F. Inoue, H. Watanabe, K. Ohsumi, M. Ohsugi and T. Hirano. (2017). Mitotic chromosome assembly despite nucleosome depletion in Xenopus egg extracts. Science. 356:1284-1287.
PMID: 28522692
Kamada, K., M. Su'etsugu, H. Takada, M. Miyata and T. Hirano. (2017). Overall shapes of the SMC-ScpAB complex are determined by balance between constraint and relaxation of its structural parts. Structure. 25:603-616.
PMID: 28286005
Kinoshita, K. and T. Hirano. (2017). Dynamic organization of mitotic chromosomes. Curr Opin Cell Biol. 46:46-53.
PMID: 28214612
Shintomi, K. and T. Hirano. (2017). A sister chromatid cohesion assay using Xenopus egg extracts. Methods Mol Biol. 1515:3-21.
PMID: 27797070

2016

Yoshimura, S. H. and T. Hirano. (2016). HEAT repeats – versatile arrays of amphiphilic helices working in crowded environments? J Cell Sci. 129: 3963-3970.
PMID: 27802131
Hirano, T. (2016). Condensin-based chromosome organization from bacteria to vertebrates. Cell. 164:847-857.
PMID: 26919425

2015

Shintomi, K., T. S. Takahashi, and T. Hirano. (2015). Reconstitution of mitotic chromatids with a minimum set of purified factors. Nat Cell Biol. 17:1014-1023.
PMID: 26075356
Sutani, T., T. Sakata, R. Nakato, K. Matsuda, M. Ishibashi, D. Yamashita, Y. Suzuki, T. Hirano, M. Bando, and K. Shirahige. (2015). Condensin targets and reduces unwound DNA structure associated with transcription in mitotic chromosome condensation. Nat Commun. 6:7815.
PMID: 26204128
Houlard, M., J. Godwin, J. Metson, J. Lee, T. Hirano and K. Nasmyth. (2015). Condensin confers the longitudinal rigidity of chromosomes. Nat Cell Biol. 17:771-781.
PMID: 25961503
Hirano, T. (2015). Chromosome dynamics during mitosis. Cold Spring Harb. Perspect. Biol. doi:10.1101/cshperspect.a015792.
PMID: 25722466
Kinoshita, K., T. J. Kobayashi and T. Hirano. (2015). Balancing acts of two HEAT subunits of condensin I support dynamic assembly of chromosome axes. Dev Cell. 33:94-106.
PMID: 25850674
Arroyo, M., M. Trimborn, A. Sanchez, T. Hirano, H. Neitzel and J. A. Marchal. (2015). Chromosome structure deficiencies in MCPH1 syndrome. Chromosoma. 124:491-501.
PMID: 25845520

2014

Nishide, K. and T. Hirano. (2014). Overlapping and non-overlapping functions of condensins I and II in neural stem cell divisions. PLoS Genet. 10:e1004847. 著者による解説
PMID: 25474630
Fujiwara, T. and T. Hirano. (2014). Specific contribution of condensin II to sister centromere resolution in Cyanidioschyzon merolae. In Atlas of Plant Cell Structure, Noguchi et al. ed. Springer, pp. 20.
Hirano, T. (2014). Condensins and the evolution of torsion-mediated genome organization. Trends Cell Biol. 24:727-733.
PMID: 25092191
Nakagawa, S. and T. Hirano. (2014). Gathering around Firre. Nat Struct Mol Biol 21:207-208.
PMID: 24599247

2013

Fujiwara, T., M. Ohnuma, M. Yoshida, T. Kuroiwa and T. Hirano. (2013). Gene targeting in the red alga Cyanidioschyzon merolae: single- and multi-copy insertions using authentic and chimeric selection markers. PLoS One. 8:e73608.
PMID: 24039997
Fujiwara, T., K. Tanaka, T. Kuroiwa and T. Hirano. (2013). Spatiotemporal dynamics of condensins I and II: evolutionary insights from the primitive red alga Cyanidioschyzon merolae. Mol Biol Cell. 24:2515-2527.
PMID: 23783031
Kamada, K., M. Miyata and T. Hirano. (2013). Molecular basis of SMC ATPase activation: role of internal structural changes of the regulatory subcomplex ScpAB. Structure. 21:581-594.
PMID: 23541893
Ono, T., D. Yamashita and T. Hirano. (2013). Condensin II initiates sister chromatid resolution during S phase. J Cell Biol. 200:429-441.
PMID: 23401001

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2012

Shiheido, H., Y. Naito, H. Kimura, H. Genma, H. Takashima, T. Ono, T. Hirano, W. Du, T. Yamada, N. Doi, S. Iijima, Y. Hattori and H. Yanagawa. (2012). An anilinoquinazoline derivative inhibits tumor growth through interaction with hCAP-G2, a subunit of condensin II. PLoS One. 7:e44889.
PMID: 23028663
Hirano, T. (2012). Chromosome territories meet a condensin. PLoS Genet. 8:e1002939.
PMID: 22956917
Hirano, T. (2012). Condensins: universal organizers of chromosomes with diverse functions. Genes Dev. 26:1659-1678.
PMID: 22855829

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2011

Yamashita, D., K. Shintomi, T. Ono, I. Gavvovidis, D. Schindler, H. Neitzel, M. Trimborn and T. Hirano. (2011). MCPH1 regulates chromosome condensation and shaping as a composite modulator of condensin II. J Cell Biol. 194:841-854.
PMID: 21911480
Lee, J., S. Ogushi, M. Saitou and T. Hirano. (2011). Condensin I and II are essential for construction of bivalent chromosomes in mouse oocytes. Mol Biol Cell. 22:3465-3477.
PMID: 21795393
Shintomi, K. and T. Hirano. (2011). The relative ratio of condensin I to II determines chromosome shapes. Genes Dev. 25:1464-1469.
PMID: 21715560
Lee, J. and T. Hirano. (2011). RAD21L, a novel cohesin subunit implicated in linking homologous chromosomes in mammalian meiosis. J Cell Biol. 192:263-276.
PMID: 21242291

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2010

Gavvovidis, I., C. Pöhlmann, J. A. Marchal, M. Stumm, D. Yamashita, T. Hirano, D. Schindler, H. Neitzel and M. Trimborn. (2010). MCPH1 patient cells exhibit delayed release from DNA damage-induced G2/M checkpoint arrest. Cell Cycle. 9:4893-4899.
PMID: 21150325
Hirano, T. (2010). How to separate entangled sisters: interplay between condensin and decatenase. Proc Natl Acad Sci USA. 107:18749-18750.
PMID: 20962275
Shintomi, K. and T. Hirano. (2010). Sister chromatid resolution: a cohesion releasing network and beyond. Chromosoma. 119:459-467.
PMID: 20352243

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2009

Takemoto, A., K. Maeshima, T. Ikehara, K. Yamaguchi, A. Murayama, S. Imamura, N. Imamoto, S. Yokoyama, T. Hirano, Y. Watanabe, F. Hanaoka, J. Yanagisawa and K. Kimura. (2009). The chromosomal association of condensin II is regulated by a noncatalytic function of PP2A. Nat Struct Mol Biol. 16:1302-1308.
PMID: 19915589
Shintomi, K. and T. Hirano. (2009). Releasing cohesin from chromosome arms in early mitosis: opposing actions Wapl-Pds5 and Sgo1. Genes Dev. 23:2224-2236.
PMID: 19696148
Hirano, T. (2009). Let's play polo in the field of condensation. Mol Cell. 34:399-401.
PMID: 19481518

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2007

Shintomi, K. and T. Hirano. (2007). How are cohesin rings opened and closed? Trends Biochem Sci. 32:154–157.
PMID: 17320400
Onn, I., N. Aono, M. Hirano and T. Hirano. (2007). Reconstitution and subunit geometry of human condensin complexes. EMBO J. 26:1024–1034.
PMID: 17268547

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2006

Gandhi, R., P. J. Gillespie and T. Hirano. (2006). Human Wapl is a cohesin-binding protein that promotes sister chromatid resolution in mitotic prophase. Curr Biol. 16:2406-2417.
PMID: 17112726
Hirano, T. (2006). At the heart of the chromosome: SMC proteins in action. Nat Rev Mol Cell Biol. 7:311-322.
PMID: 16633335
Trimborn, M., D. Shindler, H. Neitzel and T. Hirano. (2006). Misregulated chromosome condensation in MCPH1 microcephaly is mediated by condensin II. Cell Cycle. 5:322-326.
PMID: 16434882
Hirano, M. and T. Hirano. (2006). Opening closed arms: long-distance activation of SMC ATPase by hinge-DNA interactions. Mol Cell. 21:175-186.
PMID: 16427008

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2005

Losada, A. and T. Hirano. (2005). Dynamic molecular linkers of the genome: the first decade of SMC proteins. Genes Dev. 19:1269-1287.
PMID: 15937217
Losada, A., T. Yokochi and T. Hirano. (2005). Functional contribution of Pds5 to cohesin-mediated cohesion in human cells and Xenopus egg extracts. J Cell Sci. 118:2133-2141.
PMID: 15855230
Hirano, T. (2005). SMC proteins and chromosome mechanics: from bacteria to humans. Philos Trans R Soc Lond B Biol Sci. 360:507-514.
PMID: 15897176
Hirano, T. (2005). Condensins: organizing and segregating the genome. Curr Biol. 15:R265-R275.
PMID: 15823530
Hirano, T. (2005). Holding sisters for repair. Nature. 433:467-468.
PMID: 15690020

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2004

Kireeva, N., M. Lakonishok, I. Kireev, T. Hirano and A. S. Belmont. (2004). Visualization of early chromosome condensation: a hierarchical folding, axial glue model of chromosome structure. J Cell Biol. 166:775-785.
PMID: 15353545
Gillespie, P. J. and T. Hirano. (2004). Scc2 couples replication licensing to sister chromatid cohesion in Xenopus egg extracts. Curr Biol. 14:1598-1603.
PMID: 15341749
Hirano, M. and T. Hirano. (2004). Positive and negative regulation of SMC-DNA interactions by ATP and accessory proteins. EMBO J. 23:2664-2673.
PMID: 15175656
Ono, T., Y. Fang, D. L. Spector and T. Hirano. (2004). Spatial and temporal regulation of condensins I and II in mitotic chromosome assembly in human cells. Mol Biol Cell. 15:3296-3308.
PMID: 15146063
Strick, T., T. Kawaguchi and T. Hirano. (2004). Real-time detection of single-molecule DNA compaction by condensin I. Curr Biol. 14:874-880.
PMID: 15186743
Almagro, S., D. Riveline, T. Hirano, B. Houchmandzadeh and S. Dimitrov. (2004). The mitotic chromosome: an assembly of rigid elastic axes, organized by SMC proteins and surrounded by a soft chromatin envelope. J Biol Chem. 279:5118-5126.
PMID: 14660618
Hirano, T. (2004). Chromosome shaping by two condensins. Cell Cycle. 3:26-28.
PMID: 14657659

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2003

Ono, T., A. Losada, M. Hirano, M. P. Myers, A. F. Neuwald and T. Hirano. (2003). Differential contributions of condensin I and condensin II to mitotic chromosome architecture in vertebrate cells. Cell. 115:109-121.
PMID: 14532007
Almagro, S., S. Dimitrov, T. Hirano, M. Vallade and D. Riveline. (2003). Individual chromosomes as viscoelastic copolymers. Europhys. Lett. 63:908-914.
Swedlow, J. R. and T. Hirano. (2003). The making of the mitotic chromosome: modern insights into classical questions. Mol Cell. 11:557-569.
PMID: 12667441
Cuvier, O. and T. Hirano. (2003). A role of topoisomerase II in linking DNA replication to chromosome condensation. J Cell Biol. 160:645-655.
PMID: 12604590

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2002

Losada, A., M. Hirano and T. Hirano. (2002). Cohesin release is required for sister chromatid resolution, but not for condensin-mediated compaction, at the onset of mitosis. Genes Dev. 16:3004-3016.
PMID: 12464631
Hirano, M. and T. Hirano. (2002). Hinge-mediated dimerization of SMC protein is essential for its dynamic interaction with DNA. EMBO J. 21:5733-5744.
PMID: 12411491
Gillespie, P. J. and T. Hirano. (2002). SMC proteins. Curr Biol. 12:R513.
PMID: 12176371
Bazett-Jones, D. P., K. Kimura and T. Hirano. (2002). Efficient supercoiling of DNA by a single condensin complex as revealed by electron spectroscopic imaging. Mol Cell. 9:1183-1190.
PMID: 12086616
Eide, T., C. Carlson, K. A. Tasken, T. Hirano, K. Tasken and P. Collas. (2002). Distinct but overlapping domains of AKAP95 are implicated in chromosome condensation and condensin targeting. EMBO Rep. 3:426-432.
PMID: 11964380
Hirano, T. (2002). The ABCs of SMC proteins: two-armed ATPases for chromosome condensation, cohesion and repair. Genes Dev. 16:399-414.
PMID: 11850403
Anderson, D. E., A. Losada, H. P. Erickson and T. Hirano. (2002). Condensin and cohesin display different arm conformations with characteristic hinge angles. J Cell Biol. 156:419-424.
PMID: 11815634
MacCallum, D. E., A. Losada, R. Kobayashi and T. Hirano. (2002). ISWI remodeling complexes in Xenopus egg extracts: identification as major chromosomal components that are regulated by INCENP-aurora B. Mol Biol Cell. 13:25-39.
PMID: 11809820

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2001

Losada, A. and T. Hirano. (2001). Shaping the metaphase chromosome: coordination of cohesion and condensation. BioEssays. 23:924-935.
PMID: 11598959
Hirano, M., D. E. Anderson, H. P. Erickson and T. Hirano. (2001). Bimodal activation of SMC ATPase by intra- and inter-molecular interactions. EMBO J. 20:3238-3250.
PMID: 11406600
Losada, A. and T. Hirano. (2001). Intermolecular DNA interactions stimulated by the cohesin complex in vitro: implications for sister chromatid cohesion. Curr Biol. 11:268-272.
PMID: 11250156
Kimura, K., O. Cuvier and T. Hirano. (2001). Chromosome condensation by a human condensin complex in Xenopus egg extracts. J Biol Chem. 276:5417-5420.
PMID: 11136719

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2000

Kimura, K. and T. Hirano. (2000). Dual roles of the 11S regulatory subcomplex in condensin functions. Proc Natl Acad Sci USA. 97:11972-11977.
PMID: 11027308
Neuwald, A. F. and T. Hirano. (2000). HEAT repeats associated with condensins, cohesins and other chromosome-related complexes. Genome Res. 10:1445-1452.
PMID: 11042144
Losada, A. and T. Hirano. (2000). New light on sticky sisters. Curr. Biol. 10:R615.
PMID: 10996082
Hirano, T. (2000). Chromosome cohesion, condensation and separation. Annu Rev Biochem. 69:115-144.
PMID: 10966455
Losada, A., T. Yokochi, R. Kobayashi and T. Hirano. (2000). Identification and characterization of SA/Scc3p subunits in the Xenopus and human cohesin complexes. J Cell Biol. 150:405-416.
PMID: 10931856

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独立行政法人理化学研究所平野染色体ダイナミクス研究室　〒351-0198 埼玉県和光市広沢2-1

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