Keishi Shintomi, Ph. D.
Education
| 1996-2000 | Tokyo Institute of Technology, Tokyo/Yokohama, Japan. B. Sc., Biology, 2000 |
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| 2000-05 | Tokyo Institute of Technology, Yokohama, Japan. Ph. D., Cell Biology, 2005 |
Research and Professional Experience
| 2005-07 | Postdoctoral Fellow, Cold Spring Harbor Laboratory, U.S.A. |
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| 2007-11 | Postdoctoral Fellow, RIKEN, Japan. |
| 2011-18 | Research Scientist, RIKEN, Japan. |
| 2018-present | Senior Research Scientist, RIKEN, Japan. |
Awards and Professional Activities
| 2007-10 | Fellowship for Special Postdoctoral Researcher at RIKEN |
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| 2008 | RIKEN Incentive Research Grant |
| 2010 | RIKEN Incentive Research Award |
| 2003- | Member, The Molecular Biology Society of Japan |
Publication List
- Shintomi, K., Y. Masahara-Negishi, M. Shima, S. Tane and T. Hirano. (2024). Recombinant cyclin B-Cdk1-Suc1 capable of multi-site mitotic phosphorylation in vitro. PLoS One. 19:e0299003.
PMID: 38527022 - Yoshida, M. M., K. Kinoshita, K. Shintomi, Y. Aizawa and T. Hirano. (2024). Regulation of condensin II by self-suppression and release mechanisms. Mol Biol Cell. 35:ar21.
PMID: 38088875 - Sun, M., H. Amiri, A. B. Tong, K. Shintomi, T. Hirano, C. Bustamante and R. Heald. (2023). Monitoring the compaction of single DNA molecules in Xenopus egg extract in real time. Proc Natl Acad Sci USA. 120:e2221309120.
PMID: 36917660 - Fukuda, Y., K. Shintomi, K. Yamaguchi, Y. Fujiwara and Y. Okada. (2023). Solubilization of mouse sperm chromatin for sequencing analyses using a chaperon protein. Methods Mol Biol. 2577:161-173.
PMID: 36173572 - Tane, S., K. Shintomi, K. Kinoshita, Y. Tsubota, M. M. Yoshida, T. Nishiyama and T. Hirano. (2022). Cell cycle-specific loading of condensin I is regulated by the N-terminal tail of its kleisin subunit. eLife. 11:e84694.
PMID: 36511239 - Yoshida, M. M., K. Kinoshita, Y. Aizawa, S. Tane, D. Yamashita, K. Shintomi and T. Hirano. (2022). Molecular dissection of condensin II-mediated chromosome assembly using in vitro assays. eLife. 11:e78984.
PMID: 35983835 - Shintomi, K. (2022). Making mitotic chromosomes in a test tube. Epigenomes. 6:20.
PMID: 35893016 - Kinoshita, K., Y. Tsubota, S. Tane, Y. Aizawa, R. Sakata, K. Takeuchi, K. Shintomi, T. Nishiyama and T. Hirano. (2022). A loop extrusion-independent mechanism contributes to condensin I-mediated chromosome shaping. J Cell Biol. 221:e202109016.
PMID: 35045152 - Shintomi, K. and T. Hirano. (2021). Guiding functions of the C-terminal domain of topoisomerase IIα advance mitotic chromosome assembly. Nat Commun. 12:2917.
PMID: 34006877 - Shintomi, K. and T. Hirano. (2018). Reconstitution of mitotic chromatids in vitro. Curr Protoc Cell Biol. 79:e48.
PMID: 29924489 - Shintomi, K. and T. Hirano. (2017). Mitotic chromosome assembly in vitro: functional cross talk between nucleosomes and condensins. Cold Spring Harb Symp Quant Biol. pii: 033639. doi: 10.1101/sqb.2017.82.033639.
PMID: 29118204 - Shintomi, K., F. Inoue, H. Watanabe, K. Ohsumi, M. Ohsugi and T. Hirano. (2017). Mitotic chromosome assembly despite nucleosome depletion in Xenopus egg extracts. Science. 356:1284-1287.
PMID: 28522692 - Shintomi, K. and T. Hirano. (2017). A sister chromatid cohesion assay using Xenopus egg extracts. Methods Mol Biol. 1515:3-21.
PMID: 27797070 - Shintomi, K., T. S. Takahashi, and T. Hirano. (2015). Reconstitution of mitotic chromatids with a minimum set of purified factors. Nat Cell Biol. 17:1014-1023.
PMID: 26075356 - Yamashita, D., K. Shintomi, T. Ono, I. Gavvovidis, D. Schindler, H. Neitzel, M. Trimborn and T. Hirano. (2011). MCPH1 regulates chromosome condensation and shaping as a composite modulator of condensin II. J Cell Biol. 194:841-854.
PMID: 21911480 - Shintomi, K. and T. Hirano. (2011). The relative ratio of condensin I to II determines chromosome shapes. Genes Dev. 25:1464-1469.
PMID: 21715560 - Shintomi, K. and T. Hirano. (2010). Sister chromatid resolution: a cohesion releasing network and beyond. Chromosoma. 119:459-467.
PMID: 20352243 - Shintomi, K. and T. Hirano. (2009). Releasing cohesin from chromosome arms in early mitosis: opposing actions Wapl-Pds5 and Sgo1. Genes Dev. 23:2224-2236.
PMID: 19696148 - Shintomi, K. and T. Hirano. (2007). How are cohesin rings opened and closed? Trends Biochem Sci. 32:154-157.
PMID: 17320400 - Shintomi K., M. Iwabuchi, H. Saeki, K. Ura, T. Kishimoto and K. Ohsumi. (2005). Nucleosome assembly protein-1 is a linker histone chaperone in Xenopus eggs. Proc Natl Acad Sci U S A. 102:8210-8215.
PMID: 15928086
