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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC29A10.10c
Gene ID 51/F01
Gene synonyms/obsolete
Gene product tRNA-splicing endonuclease positive effector
Entry clone Cloned
ORF length (unspliced) 5835
ORF length (spliced)
Entry clone length 5835
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBC29A10.10.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAAAATGACGAGGCTTT
Rev primer name SPBC29A10.10.Rv
Rev primer SEQ AGAAAGCTGGGTACTTCCTTTTGAAATGCTCA
Amino acid length 1944
Molecular weight 222.2
Isoelectric point (calc.) 8.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LQVAVRECLFL/LDDFKSLCL/LVTVSYWLKL/LKSLRHLPL
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 2 images) See all images

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