| Gene name |
SPAC977.09c |
| Gene ID |
51/E06 |
| Gene synonyms/obsolete |
|
| Gene product |
lysophospholipase
(putative); telomeric duplication (c1348); similar to Sp
SPBC1348.10C and SPAC1A6.03c and SPCC1450.09c and SPAC1786.02
and SPAC1A6.04c |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2086 |
| ORF length (spliced) |
2022 |
| Entry clone length |
2086 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC977.09.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTATGTCAACTATATTGG |
| Rev primer name |
SPAC977.09.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACGGATTTTCTTTCGCTTCG |
| Amino acid length |
673 |
| Molecular weight |
74.5 |
| Isoelectric point (calc.) |
4.4 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LFAFVQISLTL |
| Localization (YFP) |
ER;
cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |