Gene name |
SPAC977.09c |
Gene ID |
51/E06 |
Gene synonyms/obsolete |
|
Gene product |
lysophospholipase
(putative); telomeric duplication (c1348); similar to Sp
SPBC1348.10C and SPAC1A6.03c and SPCC1450.09c and SPAC1786.02
and SPAC1A6.04c |
Entry clone |
Cloned |
ORF length (unspliced) |
2086 |
ORF length (spliced) |
2022 |
Entry clone length |
2086 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC977.09.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTATGTCAACTATATTGG |
Rev primer name |
SPAC977.09.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACGGATTTTCTTTCGCTTCG |
Amino acid length |
673 |
Molecular weight |
74.5 |
Isoelectric point (calc.) |
4.4 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LFAFVQISLTL |
Localization (YFP) |
ER;
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |