| Gene name |
SPBC29A10.08 |
| Gene ID |
51/D07 |
| Gene synonyms/obsolete |
|
| Gene product |
GPI-anchored protein;
glycoprotein; glycosidase; involved in cell wall biosynthesis;
involved in the regulation of the crosslinking of
beta-1,6-glucans in the cell wall; similar to Sp SPAC19B12.02c
|
| Entry clone |
Cloned |
| ORF length (unspliced) |
1380 |
| ORF length (spliced) |
|
| Entry clone length |
1380 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC29A10.08.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTCTCCTTTACCAAATT |
| Rev primer name |
SPBC29A10.08.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATGCCTTAAGCTCACCAACA |
| Amino acid length |
459 |
| Molecular weight |
50.6 |
| Isoelectric point (calc.) |
5.1 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
ER |
| Comments for localization |
too dark to
photo |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Zeiss |