Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPCC1739.15
Gene ID 51/D06
Gene synonyms/obsolete wtf21; wtf3
Gene product wtf element; no apparent orthologs
Entry clone Cloned
ORF length (unspliced) 1328
ORF length (spliced) 990
Entry clone length 1328
No. of intron 5
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPCC794.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAGAATAATTACACTTC
Rev primer name SPCC1739.15.Rv
Rev primer SEQ AGAAAGCTGGGTAGACTTCGCTTTCAACATCC
Amino acid length 329
Molecular weight 36.8
Isoelectric point (calc.) 4.9
Signal SEQ
No. of transmembrane domain 5
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LAKLAKVLLL/LFIMGNVLFL
Localization (YFP) vacuole; ambiguous structure
Comments for localization too dark to photo
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 2 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.