Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAPB17E12.02
Gene ID 51/C05
Gene synonyms/obsolete yip12; yip1; yip1-b
Gene product structural similarity with SMN protein; Yip1p structural similarity with SMN protein; involved in spliceosomal snRNP biogenesis; similar to Sp yip11 (paralog); duplicated in Sp; telomerase associated(implicated); no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 887
ORF length (spliced) 708
Entry clone length 887
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAPB17E12.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCCCTCGAAAAGAAAAAG
Rev primer name SPAPB17E12.02.Rv
Rev primer SEQ AGAAAGCTGGGTATGTTTGAAAGAGATCTTTT
Amino acid length 235
Molecular weight 26.9
Isoelectric point (calc.) 4.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 2 images) See all images

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