| Gene name |
SPBC18H10.18c |
| Gene ID |
51/C01 |
| Gene synonyms/obsolete |
|
| Gene product |
hypothetical protein;
sequence orphan; recent duplication; similar to Sp
SPCC1682.06 |
| Entry clone |
Cloned |
| ORF length (unspliced) |
774 |
| ORF length (spliced) |
729 |
| Entry clone length |
774 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC18H10.18.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCAGACGAAAAGTTACC |
| Rev primer name |
SPBC18H10.18.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGCTGCTGCCCGATTCTACA |
| Amino acid length |
242 |
| Molecular weight |
27 |
| Isoelectric point (calc.) |
6.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
2 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LPFILTLLL/LAHIILSLLL |
| Localization (YFP) |
cytoplasmic dots |
| Comments for localization |
aggregates by over
expression; vacuole? |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |