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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC977.08
Gene ID 51/B09
Gene synonyms/obsolete
Gene product short chain dehydrogenase (putative); telomeric duplication; similar to Sp SPBC1348.09; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 711
ORF length (spliced)
Entry clone length 711
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC977.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCGTGAACCCAAGAATGC
Rev primer name SPAC977.08.Rv
Rev primer SEQ AGAAAGCTGGGTAAAGACCCATTTGTTTTCGG
Amino acid length 236
Molecular weight 26.4
Isoelectric point (calc.) 6.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 2 images) See all images

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