| Gene name |
SPBP16F5.03c |
| Gene ID |
50/A01 |
| Gene synonyms/obsolete |
|
| Gene product |
putative
phosphatidylinositol kinase; similarity phosphatidylinositol
kinase; SAGA complex; FAT domain; FATC domain; similar to Sp
SPAC1F5.11c (paralog) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
11100 |
| ORF length (spliced) |
|
| Entry clone length |
11100 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
992G:A / 2323T:C /
3096T:G / 7179T:C / 9318A:G / 1031?A:G / 1048?A:G |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBP16F5.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCAGCTTGCTCTATCCCA |
| Rev primer name |
SPBP16F5.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACAGCCAAGGAGCCCATAAG |
| Amino acid length |
3699 |
| Molecular weight |
422.3 |
| Isoelectric point (calc.) |
7.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LPLLAPLVL/LLEMLATLFL/LVDNLALLHI/LYAELCLTLPI/LLDSIMPDLLI/LEWLIVLDL/LQSIKMLDL/LSVVDLLLPI/LEGLSRLLRL/LWAKLPRLVI/LTFLIKFLSL/LEQIILLLL/LQKSVSQPLAI/LARVLWLLSL/LTRYLNDALAL |
| Localization (YFP) |
no apparent
signal |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
DeltaVision |