Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAPB1E7.07
Gene ID 49/H09
Gene synonyms/obsolete
Gene product glutamate synthase; involved in glutamate biosynthesis
Entry clone Cloned
ORF length (unspliced) 6336
ORF length (spliced)
Entry clone length 6336
No. of intron 0
Sequence status Finished
Sequence results 294T:C / 1713T:C / 1875T:C / 2246T:C / 3087T:C / 3865A:G / 4785T:A / 5421T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAPB1E7.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCTGTCTTATCTTCAGT
Rev primer name SPAPB1E7.07.Rv
Rev primer SEQ AGAAAGCTGGGTAACAATTAACAGTGCGCTTT
Amino acid length 2111
Molecular weight 232.8
Isoelectric point (calc.) 6.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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