| Gene name |
SPAPB1E7.07 |
| Gene ID |
49/H09 |
| Gene synonyms/obsolete |
|
| Gene product |
glutamate synthase;
involved in glutamate biosynthesis |
| Entry clone |
Cloned |
| ORF length (unspliced) |
6336 |
| ORF length (spliced) |
|
| Entry clone length |
6336 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
294T:C / 1713T:C /
1875T:C / 2246T:C / 3087T:C / 3865A:G / 4785T:A /
5421T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAPB1E7.07.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCTGTCTTATCTTCAGT |
| Rev primer name |
SPAPB1E7.07.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAACAATTAACAGTGCGCTTT |
| Amino acid length |
2111 |
| Molecular weight |
232.8 |
| Isoelectric point (calc.) |
6.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |