| Gene name |
SPBC713.02c |
| Gene ID |
49/G03 |
| Gene synonyms/obsolete |
ubp15; ubp21;
ubpd |
| Gene product |
ubiquitin C-terminal
hydrolase activity; peptidase family C19; MATH domain; similar
to Sp SPCC188.08c (paralog); overexpression can partially
suppress a mutation in Prp4p; involved in regulating levels of
Prp4p; double mutant ubp21 delta/ubp22 delta lethal |
| Entry clone |
Cloned |
| ORF length (unspliced) |
3543 |
| ORF length (spliced) |
3390 |
| Entry clone length |
3543 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
277C:G / 802T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC713.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTAAGTTTACTTTTTTA |
| Rev primer name |
SPBC713.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTTCATTCTTATTGCTTGG |
| Amino acid length |
1129 |
| Molecular weight |
130.8 |
| Isoelectric point (calc.) |
5.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LPNVLQLQL |
| Localization (YFP) |
nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |