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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC688.01
Gene ID 49/F09
Gene synonyms/obsolete SPAC589.12
Gene product hypothetical protein; involved in sensitivity to certain drugs
Entry clone Cloned
ORF length (unspliced) 3100
ORF length (spliced) 2916
Entry clone length 3100
No. of intron 1
Sequence status Finished
Sequence results 72T:C / 275A:G / 1308A:G / 2499G:A
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC688.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACAGAAAAAACAAGCTC
Rev primer name SPAC688.01.Rv
Rev primer SEQ AGAAAGCTGGGTAATCATAGTACCACGGCTCG
Amino acid length 971
Molecular weight 110
Isoelectric point (calc.) 7.6
Signal SEQ
No. of transmembrane domain 19
NLS position (Columbia Univ. Bioinformatics Center) 665
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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