Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAPJ730.01
Gene ID 48/G03
Gene synonyms/obsolete win1; SPAC1006.09; SPAC1250.06c
Gene product serine/threonine protein kinase; MAP kinase kinase kinase (MAPKKK); involved in mitotic control; Spc1 SAPK cascade; activator of Wis1p by phosphorylation in response to high osmolarity; G2/M phase transition
Entry clone Cloned directly into pDUAL-FFH1
ORF length (unspliced) 4311
ORF length (spliced)
Entry clone length 4311
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAPJ730.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAAAATATTTTGGATCC
Rev primer name SPAPJ730.01.Rv
Rev primer SEQ AGAAAGCTGGGTAAAGTTCCAACGGAGCACCA
Amino acid length 1436
Molecular weight 163.2
Isoelectric point (calc.) 6.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LIKEFLNLRL
Localization (YFP) no expression clone
Comments for localization
Effect of LMB on protein localization not determined
Microscope used for observation

Image information
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