| Gene name |
SPBC887.12 |
| Gene ID |
48/E11 |
| Gene synonyms/obsolete |
|
| Gene product |
P-type ATPase; P4
type; aminophospholipid translocase |
| Entry clone |
Cloned |
| ORF length (unspliced) |
3777 |
| ORF length (spliced) |
|
| Entry clone length |
3777 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
710A:G / 993A:C /
1246T:C / 2848A:C |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC887.12.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCCCGAGATGTAGATAA |
| Rev primer name |
SPBC887.12.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAACGCATTTCACCGTATGCT |
| Amino acid length |
1258 |
| Molecular weight |
142.3 |
| Isoelectric point (calc.) |
5.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
8 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LYTFDATLKL/LIHQFLLVLSI |
| Localization (YFP) |
ER |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |