| Gene name |
SPBC36.07 |
| Gene ID |
48/E10 |
| Gene synonyms/obsolete |
|
| Gene product |
human ikappab kinase
complex associated protein; RNA polymerase II (elongator
subunit); Pol II transcription elongation factor activity;
transcription elongation factor complex |
| Entry clone |
Cloned |
| ORF length (unspliced) |
3762 |
| ORF length (spliced) |
|
| Entry clone length |
3762 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC36.07.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAAAATTTGGTAACACA |
| Rev primer name |
SPBC36.07.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATATTAAAATACTCAATTTT |
| Amino acid length |
1253 |
| Molecular weight |
141.6 |
| Isoelectric point (calc.) |
5.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LGEPMAICLSL/LYRVDYLDL/LYDLKLALLI |
| Localization (YFP) |
cytosol |
| Comments for localization |
one nuclear dot by
over expression? |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |