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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC167.01
Gene ID 48/B07
Gene synonyms/obsolete
Gene product serine/threonine protein kinase; involved in unfolded protein response
Entry clone Cloned
ORF length (unspliced) 3269
ORF length (spliced) 3219
Entry clone length 3269
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC167.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAGAGTTTGAAAGGGCG
Rev primer name SPAC167.01.Rv
Rev primer SEQ AGAAAGCTGGGTAATACTCTAGATAACGTTTA
Amino acid length 1072
Molecular weight 121.2
Isoelectric point (calc.) 6.6
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain 2
NLS position (Columbia Univ. Bioinformatics Center) 568
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LRKFVFFSLLI
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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