| Gene name |
SPCC24B10.01 |
| Gene ID |
47/B07 |
| Gene synonyms/obsolete |
cdc21; mcm4;
SPCC16A11.17 |
| Gene product |
MCM complex subunit;
involved in DNA replication (initiation) (required) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2736 |
| ORF length (spliced) |
|
| Entry clone length |
2736 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPCC24B10.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCCTCTAGTCAGCAAAG |
| Rev primer name |
SPCC24B10.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATCAGTCTGTGCAATTGAA |
| Amino acid length |
911 |
| Molecular weight |
101.5 |
| Isoelectric point (calc.) |
7.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
443 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LISIKGLVL |
| Localization (YFP) |
no transformant |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
|