| Gene name |
SPAP27G11.12 |
| Gene ID |
47/A11 |
| Gene synonyms/obsolete |
|
| Gene product |
conserved
hypothetical; eukaryotic conserved protein; no apparent Sc
ortholog |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2675 |
| ORF length (spliced) |
2394 |
| Entry clone length |
2675 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAP27G11.12.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGTTCTCAACAGTCTAA |
| Rev primer name |
SPAP27G11.12.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAATGAGTCAAATATTTTG |
| Amino acid length |
797 |
| Molecular weight |
92.5 |
| Isoelectric point (calc.) |
7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LNTVVDYLFL/LHRVEVLRL/LVLIFCKLFI/LCCSLCYLCL/LDIFTDLSL |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |