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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC4G9.09c
Gene ID 47/A09
Gene synonyms/obsolete arg11
Gene product N-acetyl-gamma-glutamyl-phosphate reductase; acetylglutamate kinase; involved in arginine biosynthesis; involved in ornithine biosynthesis; N-acetyl-gamma-glutamyl-phosphate reductase activity; acetylglutamate kinase activity
Entry clone Cloned
ORF length (unspliced) 2658
ORF length (spliced)
Entry clone length 2658
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC4G9.09.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCTTATTGAATTGCAACA
Rev primer name SPAC4G9.09.Rv
Rev primer SEQ AGAAAGCTGGGTAATCCAAATGGATACCGGCA
Amino acid length 885
Molecular weight 97.7
Isoelectric point (calc.) 9.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 4 images) See all images

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