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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC3H7.02
Gene ID 47/A05
Gene synonyms/obsolete
Gene product SulP sulfate transporter
Entry clone Cloned
ORF length (unspliced) 2634
ORF length (spliced)
Entry clone length 2634
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBC3H7.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTTTTATCAAGAAAAT
Rev primer name SPBC3H7.02.Rv
Rev primer SEQ AGAAAGCTGGGTATGCGGCACCGTCTGAGTGC
Amino acid length 877
Molecular weight 96.3
Isoelectric point (calc.) 8.2
Signal SEQ
No. of transmembrane domain 11
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LAGAITCGLGL/LVSLFILYL/LPVSVIVLLL
Localization (YFP) cytoplasmic dots; periphery at cell tip and site of septum formation
Comments for localization bright dots by over expression
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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