| Gene name |
SPBC1773.12 |
| Gene ID |
45/H01 |
| Gene synonyms/obsolete |
|
| Gene product |
transcriptional
regulator; zinc finger protein; zf-fungal Zn(2)-Cys(6)
binuclear cluster domain |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1785 |
| ORF length (spliced) |
|
| Entry clone length |
1785 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC1773.12.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTACCAATAAAAAAAAT |
| Rev primer name |
SPBC1773.12.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACACCAACAAACTCTCCAGT |
| Amino acid length |
594 |
| Molecular weight |
69.4 |
| Isoelectric point (calc.) |
7 |
| Signal SEQ |
|
| No. of transmembrane domain |
1 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
40 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LDKIQALTI |
| Localization (YFP) |
nucleolus>nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal |