| Gene name |
SPAC12G12.15 |
| Gene ID |
45/E01 |
| Gene synonyms/obsolete |
|
| Gene product |
Sad1-interacting
factor 3; 1 predicted transmembrane helix; similar to S.
cerevisiae YFR048W |
| Entry clone |
Cloned (also cloned in
2004 trial) |
| ORF length (unspliced) |
1632 |
| ORF length (spliced) |
1533 |
| Entry clone length |
1632 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC12G12.15.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTACTAAGGATAAACT |
| Rev primer name |
SPAC12G12.15.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATAAATTGATAAAAGGGGAA |
| Amino acid length |
510 |
| Molecular weight |
58.4 |
| Isoelectric point (calc.) |
8.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
1 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LYAVYHLPL/LYTAFREYLEI |
| Localization (YFP) |
nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |