Gene name |
SPAC12G12.15 |
Gene ID |
45/E01 |
Gene synonyms/obsolete |
|
Gene product |
Sad1-interacting
factor 3; 1 predicted transmembrane helix; similar to S.
cerevisiae YFR048W |
Entry clone |
Cloned (also cloned in
2004 trial) |
ORF length (unspliced) |
1632 |
ORF length (spliced) |
1533 |
Entry clone length |
1632 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC12G12.15.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTACTAAGGATAAACT |
Rev primer name |
SPAC12G12.15.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATAAATTGATAAAAGGGGAA |
Amino acid length |
510 |
Molecular weight |
58.4 |
Isoelectric point (calc.) |
8.6 |
Signal SEQ |
|
No. of transmembrane domain |
1 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LYAVYHLPL/LYTAFREYLEI |
Localization (YFP) |
nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |