| Gene name |
SPAC17C9.08 |
| Gene ID |
44/A10 |
| Gene synonyms/obsolete |
pnu1; nuc1 |
| Gene product |
endonuclease; involved
in RNA degradation; involved in the decrease in cellular RNA
during nitrogen starvation (implicated) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1237 |
| ORF length (spliced) |
1008 |
| Entry clone length |
1237 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC17C9.08.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTAGTAATCTTATCAA |
| Rev primer name |
SPAC17C9.08.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAATATTTTCATAATAAAGA |
| Amino acid length |
335 |
| Molecular weight |
38 |
| Isoelectric point (calc.) |
8.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
1 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
no apparent
signal |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
DeltaVision |