| Gene name |
SPBC16A3.09c |
| Gene ID |
40/D12 |
| Gene synonyms/obsolete |
ufd1 |
| Gene product |
ubiquitin fusion
degradation protein (putative); Cdc48-Ufd1-Npl4 complex
component (putative); involved in proteasomal processing;
involved in the recognition of polyubiquitin-tagged
proteins |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1360 |
| ORF length (spliced) |
1029 |
| Entry clone length |
1360 |
| No. of intron |
4 |
| Sequence status |
Finished |
| Sequence results |
99T:C / 430T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC16A3.09.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGCGGCAGCTTTTTCTC |
| Rev primer name |
SPBC16A3.09.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGCATCAATATCGATTGGG |
| Amino acid length |
342 |
| Molecular weight |
38.2 |
| Isoelectric point (calc.) |
4.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LPYWMMTTLSL |
| Localization (YFP) |
nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |