| Gene name |
SPBC1A4.01 |
| Gene ID |
40/A07 |
| Gene synonyms/obsolete |
apc10;
SPBC1E8.06 |
| Gene product |
anaphase-promoting
complex (APC); regulators of the APC-cyclosome; involved in
cyclin degradation (required); involved in metaphase-anaphase
transition (required) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
699 |
| ORF length (spliced) |
570 |
| Entry clone length |
699 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
301A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC1A4.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCGCAAATTCGACAAGA |
| Rev primer name |
SPBC1A4.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACCGTAATTGGTTTCTACTT |
| Amino acid length |
189 |
| Molecular weight |
21.4 |
| Isoelectric point (calc.) |
6.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |