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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC550.14
Gene ID 39/D01
Gene synonyms/obsolete
Gene product hypothetical KH domain RNA-binding protein RNA-binding protein; KH domain; involved in control of mitotic chromosome transmission
Entry clone Cloned
ORF length (unspliced) 3840
ORF length (spliced)
Entry clone length 3840
No. of intron 0
Sequence status Finished
Sequence results 297A:T / 1125T:C / 1629G:A / 3502C:T / 3639T:C / 3671G:A
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC550.14.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGACTCATATGATTTTCA
Rev primer name SPCC550.14.Rv
Rev primer SEQ AGAAAGCTGGGTACTCTTGATTTTGGTTTTCT
Amino acid length 1279
Molecular weight 141.2
Isoelectric point (calc.) 6.8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LRTTIPTMLPI
Localization (YFP) cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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