| Gene name |
SPCC645.06c |
| Gene ID |
39/C12 |
| Gene synonyms/obsolete |
rgf3 |
| Gene product |
hypothetical Rho1
GDP-GTP exchange protein; non-essential; interacts genetically
with septins; rho1 gdp-GTP exchange protein; RhoGEF;
pleckstrin homology domain; similar to Sp SPCC645.07 and and
SPAC1006.06; divergently orientated to rgf1 |
| Entry clone |
Cloned |
| ORF length (unspliced) |
3828 |
| ORF length (spliced) |
|
| Entry clone length |
3828 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
865A:G / 2386T:C /
2624T:C / 3585T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC645.06.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAGCTCTCCAATGAACT |
| Rev primer name |
SPCC645.06.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATCTTCAATTATGAAGGAG |
| Amino acid length |
1275 |
| Molecular weight |
144.4 |
| Isoelectric point (calc.) |
8.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LDWLDIHLFL |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
cytosol=nucleus, partially |
| Microscope used for
observation |
Leica |