| Gene name |
SPBC2F12.03c |
| Gene ID |
38/H08 |
| Gene synonyms/obsolete |
|
| Gene product |
TPR repeat protein;
EST1 domain; similar to Sp SPBC2D10.13 |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2676 |
| ORF length (spliced) |
|
| Entry clone length |
2676 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
598A:G / 1132G:A /
2192T:C / 2256A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC2F12.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGCGCCATAGATTCATG |
| Rev primer name |
SPBC2F12.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATTATTGCTGATGTTAACC |
| Amino acid length |
891 |
| Molecular weight |
98.5 |
| Isoelectric point (calc.) |
8.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSDSVNSQLPL |
| Localization (YFP) |
cytosol; cytoplasmic
dots; periphery at site of septum formation |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
intranuclear microtubule bundle (nucleus>>cytosol;
cytoplasmic dots |
| Microscope used for
observation |
Leica |