| Gene name |
SPAC22F8.05 |
| Gene ID |
38/H06 |
| Gene synonyms/obsolete |
|
| Gene product |
alpha,alpha-trehalose-phosphate synthase; glycosyl
transferase family 20; similar to Sp SPACUNK4.16c
(paralog) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2676 |
| ORF length (spliced) |
|
| Entry clone length |
2676 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
1653T:C / 1746T:C /
2258A:G / 2654T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC22F8.05.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGAAGACAATTCATTTG |
| Rev primer name |
SPAC22F8.05.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGCACATAATGAGTTTAAA |
| Amino acid length |
891 |
| Molecular weight |
100.6 |
| Isoelectric point (calc.) |
6.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSLSMKKALTL |
| Localization (YFP) |
cytosol |
| Comments for localization |
bright large dots by
over expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |